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A comparison of tissue preparation methods for protein extraction of cocoa ( Theobroma cacao L.) pod Acta Agron. (Palmira)
Martínez-Márquez,Ascensión; Morante-Carriel,Jaime Alfredo; Bru-Martínez,Roque.
Abstract: Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable protein. Efficient methods of protein extraction are a priority to successfully apply proteomic analyses. We compared and evaluated two methods (A and B) of tissue preparation for total protein extract by phenol/SDS extraction protocol. The difference in the application of the two methods was that extensively washed dry powder of pod tissue were made in Method A, whereas that crude extract were prepared Method B. Extracted proteins were examined using one-dimensional electrophoresis (1-D). Results show that each extraction method...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocoa pod; One-dimensional electrophoresis; Phenol/SDS extraction protocols; Proteome analyses.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000200248
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